Repeated column chromatographic separation of the CH2C12 extract of Artemifia stofonifera (Asteraceae) led to the isolation of a triterpene (I), a sesquiterpene (II), two aromatlc compounds (III and IV) and a benzoquinone (V). Their structures were determined by spectroscopic means to be simiarenol (I), (1S,7S)- 1 ¥â-hydroxgermacra-4(15),5, 10(14)-triene (II), 3¡¯-methoxy-4¡¯-hydroxy-trans-cinnamaldehyde (III), vanillin (IV) and 2,6-dimethoxy-1,4-benzoquinone (V), respectively. Among these products, compound V showed significant cytotoxicity against five human tumor cell lines in vitro, A549 (non small cell lung adenocar- cinoma), SK-OV-3 (ovarian), SK-MEL-2 (skin melanoma), XF498 (CNS) and HCT15 (colon) with ED50 values ranging from 1.33¡4.22 mcg/ml.
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